Plasmid

Part:BBa_K3582500:Design

Designed by: Avadhoot Jadhav   Group: iGEM20_IISER-Pune-India   (2020-10-24)


Modified Pet28a(+) expression vector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 1725
    Illegal NgoMIV site found at 1885
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2805


Design Notes

Removed excess tags and non required regions. RFC 10 criteria satisfied.


Source

Snapgene

References